| 详细描述： |
<p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 28px;"><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">胰酶细胞消化液</span>(Trypsin Solution)<span style="font-family: 宋体;">含</span></span><span style="font-family: 'Times New Roman';"> </span><span style="font-family: 'Times New Roman';">0.25%<span style="font-family: 宋体;">胰酶（</span>Trypsin<span style="font-family: 宋体;">）</span></span><span style="font-family: 宋体;">，不含</span><span style="font-family: 'Times New Roman';">EDTA</span><span style="font-family: 宋体;">和酚红</span><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">，不含</span>Ca</span><sup><span style="font-family: 'Times New Roman'; font-size: 14px; vertical-align: super;">2<span style="font-family: 宋体;">＋</span></span></sup><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">和</span> Mg</span><sup><span style="font-family: 'Times New Roman'; font-size: 14px; vertical-align: super;">2<span style="font-family: 宋体;">＋</span></span></sup><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">。该消化液已用</span> 0.22 μm <span style="font-family: 宋体;">滤膜过滤除菌，可以直接用于培养细胞的消化或者一些组织的消化。本胰酶细胞消化液具有方便快速的特点，通常室温消化 </span>1 <span style="font-family: 宋体;">分钟左右就可以消化下大多数贴壁细胞。</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px;"><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">使用说明</span></span><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">：</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 28px;"><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">贴壁细胞的消化：</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 28px;"><span style="font-family: 'Times New Roman';">1<span style="font-family: 宋体;">、吸去培养液，用无菌的 </span>PBS<span style="font-family: 宋体;">、</span>Hanks <span style="font-family: 宋体;">液或无血清培养液洗涤细胞一次，以去除残余的血清。</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 28px;"><span style="font-family: 'Times New Roman';">2<span style="font-family: 宋体;">、加入少量胰酶细胞消化液，略盖过细胞即可，室温放置 </span>30 <span style="font-family: 宋体;">秒至 </span>2 <span style="font-family: 宋体;">分钟。不同的细胞消化时间有所不同。</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 28px;"><span style="font-family: 'Times New Roman';">3<span style="font-family: 宋体;">、显微镜下观察，细胞明显收缩，并且肉眼观察培养器皿底部发现细胞的形态发生明显的变化，或者用枪吹打细胞发现细胞刚好可以被吹打下来此时吸除胰酶细胞消化液，加入含血清的完全细胞培养液，吹打下细胞，即可直接用于后续实验。</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 28px;"><span style="font-family: 'Times New Roman';">4<span style="font-family: 宋体;">、如果发现消化不足，则加入胰酶细胞消化液重新消化。</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 28px;"><span style="font-family: 'Times New Roman';">5<span style="font-family: 宋体;">、如果发现细胞消化时间过长，未及时吹打细胞，细胞已经有部分直接从培养器皿底部脱落，直接用胰酶细胞培养液把细胞全部吹打下来。</span>1000-2000g <span style="font-family: 宋体;">离心 </span>1 min<span style="font-family: 宋体;">，沉淀细胞，尽量去除胰酶细胞消化液后，加入含血清的完全培养液重新悬浮细胞，即可用于后续实验。</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 21px;"><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">组织的消化：</span></span> <p style="margin: 0px; padding: 0px; color: #2c3e50; font-family: Avenir, Helvetica, Arial, sans-serif; font-size: 14px; text-indent: 21px;"><span style="font-family: 'Times New Roman';"><span style="font-family: 宋体;">不同的组织需要消化的时间相差很大，通常以消化后可以充分打散组织为宜。</span></span> |
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